Tuchel's decision to select a central defender in Chalobah, rather than a natural full-back replacement, is further proof Real Madrid's Alexander-Arnold remains well out of the picture.
Alexander-Arnold's fate seemed sealed as far back as August when Tuchel left him out of England's squad for World Cup qualifiers against Andorra and Serbia.
This was after Tuchel even opted to pick Alexander-Arnold's former Liverpool team-mate Curtis Jones, a central midfielder, ahead of him at right-back against Andorra in June.
Tuchel has made his concerns over Alexander-Arnold's perceived defensive frailties clear, saying: "If he wants to have this impact in the English national team then he has to take the defensive part very, very seriously.
"Because when we are talking, especially about qualifying football, and then tournament football, the one defensive error, the one moment where you are not 100% awake, can be decisive. It can be the moment where you pack your suitcases and go home."
Packing his suitcase early for a flight back to England is not on Tuchel's agenda - so one of the country's most naturally gifted footballers is overlooked again.
Alexander-Arnold was not included in Tuchel's 35-man squad for friendlies against Uruguay and Japan in March, missing out again when the coach needed to seek reinforcements.
Tuchel instead picked Arsenal's Ben White, who has not been a regular at club level and had been in self-imposed England exile since the Qatar World Cup in 2022.
Alexander-Arnold did make Tuchel's provisional 55-man World Cup squad, although that could hardly be called a ringing endorsement.
It is in sharp contrast to the faith placed in Alexander-Arnold by Lee Carsley, England's Under-21 coach who bridged the gap between Southgate's departure and Tuchel's appointment.
Carsley even used Alexander-Arnold at left-back in a 3-1 Nations League win at Finland in October 2024, crowning his performance with a superb free-kick.
Alexander-Arnold started four games out of six under Carsley but none of Tuchel's 14 - those facts speak for themselves.
As a head coach who fixates on squad togetherness, was there a worry that having such a high-profile player on the margins might lead Alexander-Arnold to provide an unintentional distraction and focus of attention?
Alexander-Arnold's latest rejection appears to be further evidence there is no way back for him with England as long as Tuchel is in charge.
